Synonyms for dussoix or Related words with dussoix
Examples of "dussoix"
) gained her first degree in Chemistry and Biology from University of Geneva (1958), followed by her doctorate in Biophysics (1964).
(1936 -2014) molecular microbiologist, was one of the discoverers of restriction enzymes during her doctoral studies, for which a Nobel prize was awarded to Werner Arber.
-Roulland was a member of the research groups of two future Nobel Prizewinners (Werner Arber (for discovery of restriction enzymes), and the group of Harold Varmus and J. Michael Bishop (for the cellular origin of retroviral oncogenes). Her contribution to these discoveries, and whether she should have had greater recognition, has been a topic of controversy. Specifically, the work on host-controlled DNA modification, which earned Arber the Nobel prize, was described in two 1962 articles where
and Arber were the sole authors. In a letter to her brother and sister-in-law, written in 1978, shortly after the award of the Nobel prize to Arber,
-Rolland states that "I am very furious, because apparently he has not even mentioned my name, and I have done half of the work for which he received the Nobel Prize" . In Arber's biographical sketch on the Nobel Prize site, it is stated that the findings "that restriction and modification were properties of the bacterial strains and acted not only on infecting bacteriophage DNA, but also on cellular DNA as manifested in conjugation experiments" were reported by himself and Daisy
for the first time to the scientific community during the First International Biophysics Congress held in Stockholm in the summer of 1961. Yet,
is not thanked and credited individually as a collaborator directly involved in the work rewarded by the Nobel prize. The text of Arber's Nobel lecture states that together with Grete Kellenberger "Daisy
, a Ph. D. student, studied the breakdown of DNA from irradiated phage λ upon infection of normal host bacteria" . In a letter to her brother (December 7, 1978),
states: "I have worked with Werner from 1959 to 1963, at which time they (Edouard) forced me to change my PhD thesis project, reportedly because I could not use work done with Werner, but in reality because Werner, after returning from the USA, in order to be paid decently had to engage himself on research on radiation, for which at the time there was more money. Since Werner was not at all interested in doing the research for which he was paid, somebody had to do it, and that somebody was me, this is why for more than a year before my departure to the USA I was not able to work on restriction, and that should not count for my thesis. In any event, I think that the Nobel Prize was awarded for the two papers published in 62".
In early 1980
-Roulland returned to Europe and worked at the Institut Pasteur in Paris on detection of mycoplasmas using PCR-based molecular methods. She was appointed Group Head of the Mycoplasma Laboratory in 1987 in the Viral Oncology Unit of Luc Montagnier. Her publications from these years focused on mycobacterium and mycoplasmas, specifically genetic and molecular characterization and the development of detection methods.
moved to Stanford University, USA, funded by a Jane Coffin Childs postdoctoral fellowship to work with Robert Lehman. She subsequently worked as Assistant Professor in Residence in the Department of Microbiology from 1968 at the University of California, San Francisco and continued to study DNA restriction and modification with Herbert W Boyer. She later worked with the research group of Harold E. Varmus on understanding how avian src protoncogenes worked. She subsequently moved to the University of California, Berkeley.
Back at the University of Geneva, Arber worked in a laboratory in the basement of the Physics Institute, where he carried out productive research and hosted "a number of first class graduate students, postdoctoral fellows and senior scientists." including Daisy Roulland
, whose work helped him to later obtain the nobel Prize. In 1965 the University of Geneva promoted him to Extraordinary Professor for Molecular Genetics. In 1971, after spending a year as a visiting professor in the Department of Molecular Biology of the University of California in Berkeley, Arber moved to the University of Basel. In Basel, he was one of the first persons to work in the newly constructed Biozentrum, which housed the departments of biophysics, biochemistry, microbiology, structural biology, cell biology and pharmacology and was thus conducive to interdisciplinary research.
She worked for her PhD with Werner Arber and Eduard Kellenberger, Swiss microbial geneticists, at the time when the barriers to infection of bacterial cells by virus (bacteriophage) first became apparent, leading to the discovery of restriction and modification enzymes that have subsequently became essential molecular biology tools. These enzymes result in cleavage of DNA by enzymes at sites characterised by specific sequences unless these are protected by prior enzymatic modification to the DNA bases. This system protects bacterial cells from viral infection. The research of Grete Kellenberger-Gujer had already demonstrated that phage DNA could be degraded by host bacterial cells. Daisy
and Werner Arber showed that this process required enzymes, resulting in two publications that paved the way for discovery and isolation of the restriction and modification enzymes involved.
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