Synonyms for mmct or Related words with mmct

conjugational              conjugative              scnt              transferessential              orit              tlowe              agrolistic              lavitrano              flavoprotein              conjugal              kreigler              transconjugation              entryscaled              pcamvcn              abaculovirus              conjugally              interchromosomal              diploidy              expressiona              ooplasmic              guyer              micronuclear              transgenesis              minichromosomes              agrolistics              polytene              congression              kriegler              cytcytoplasmic              zygotes              microchromosome              idlv              virusparental              uniparental              phasttransfer              microcellmediated              virusvv              formationnuclear              phleip              pitpnm              karyoplasts              megachromosome              meiotically              arterialgene              trnoe              cytoduction              wklynx              parthenogenesis              biorientation              cybrids             



Examples of "mmct"
The Mulanje Mountain Conservation Trust (MMCT) is a conservation trust in Malawi.
Microcell Mediated Chromosome Transfer (or MMCT) is a technique used in cell biology and genetics to transfer a chromosome from a defined donor cell line into a recipient cell line. MMCT has been in use since the 1970s and has contributed to a multitude of discoveries including tumor, metastasis and telomerase suppressor genes as well as information about epigenetics, x-inactivation, mitochondrial function and aneuploidy. MMCT follows the basic procedure where donor cells (i.e. cells providing one or more chromosomes or fragments to a recipient cell) are induced to multinucleate their chromosomes. These nuclei are then forced through the cell membrane to create microcells, which can be fused to a recipient cell line.
Metastasis suppressors were first identified using microcell-mediated chromosome transfer (MMCT), which introduces chromosomes into intact recipient cells. Chromosomes 1, 6, 7, 8, 10, 11, 12, 16 and 17 harbor metastasis suppressor genes.
The term MMCT was first used by Fournier and Ruddle in 1977. Their method was based on previous work from 1974 by Ege, Ringertz, Veomett and colleagues, synthesizing the techniques used at the time to induce multinucleation in cells, nuclear removal and cell-cell fusions. The next major step in MMCT came during the 1980s when new transfection techniques were utilized to introduce selectable markers onto chromosomes thus making it possible to select for the introduction of specific chromosomes and more easily create defined hybrids.
Procedures for MMCT differ slightly but they all require: the induction of multinucleation, enucleation (nuclear removal), and fusion. Multinucleation is usually accomplished through causing prolonged mitotic arrest by colcemid treatment. Certain cells will then "slip" out of mitosis and form multiple nuclei. These nuclei can then be removed using cytochalasin B to disrupt the cytoskeleton and centrifugation in a density gradient to force enucleation. The newly created microcells can then be fused to recipient (target) cells by exposure to poly ethylene glycol, use of Sendai virus, or electrofusion.
As well as providing protection from fires the Forestry Department and the Mulanje Mountain Conservation Trust carefully control the use of the trees. Back in the day sawing licenses could be issued by the Forestry Department, but because there was still an extensive amount of illegal pit sawing taking place, the sawing season has been closed since 2007. But of course the pit sawing did not stop. The black market for cedar wood grew, and has not been stopped, yet. All cedar wood sawn after 2007 is illegal wood, and can be confiscated by Forestry, MMCT and the Malawi Police.
Although a long-term cooling trend was well underway, there is evidence of a warm period during the Miocene when the global climate rivalled that of the Oligocene. The Miocene warming began 21 million years ago and continued until 14 million years ago, when global temperatures took a sharp drop—the Middle Miocene Climate Transition (MMCT). By 8 million years ago, temperatures dropped sharply once again, and the Antarctic ice sheet was already approaching its present-day size and thickness. Greenland may have begun to have large glaciers as early as 7 to 8 million years ago, although the climate for the most part remained warm enough to support forests there well into the Pliocene.