Synonyms for nanobodies or Related words with nanobodies
Examples of "nanobodies"
Ablynx is a biopharmaceutical company engaged in the discovery and development of
In diagnostic biosensor applications
may be used prospectively as a tool. Due to their small size, they can be coupled more densely on biosensor surfaces. In addition to their advantage in targeting less accessible epitopes, their conformational stability also leads to higher resistance to surface regeneration conditions. After immobilizing single-domain antibodies on sensor surfaces sensing human prostate-specific antigen (hPSA) were tested. The
outperformed the classical antibodies in detecting clinical significant concentrations of hPSA.
Single-domain antibodies have been tested as a new therapeutic tool against multiple targets. In mice infected with influenza A virus subtype H5N1,
directed against hemaglutinin suppressed replication of the H5N1 virus "in vivo" and reduced morbidity and mortality.
targeting the cell receptor binding domain of the virulence factors toxin A and toxin B of "Clostridium difficile" were shown to neutralize cytopathic effects in fibroblasts "in vitro".
Ablynx expects that their
might cross the blood–brain barrier and permeate into large solid tumours more easily than whole antibodies, which would allow for the development of drugs against brain cancers.
It has been shown that the extreme C-terminus of IRF-1 regulates its ability to activate transcription,
targeting this domain (MF1) are able to increase IRF-1 activity.
As an approach for photothermal therapy
binding to the HER2 antigen, which is overexpressed in breast and ovarian cancer cells, were conjugated to branched gold nanoparticles (see figure). Tumor cells were destroyed photothermally using a laser in a test environment.
To increase the crystallization probability of a target molecule,
can be used as crystallization chaperones. As auxiliary proteins, they can reduce the conformational heterogeneity by binding and stabilizing just a subset of conformational states. They also can mask surfaces interfering with the crystallization while extending regions that form crystal contacts.
The first single-domain antibodies were engineered from heavy-chain antibodies found in camelids; these are called VH fragments. Cartilaginous fishes also have heavy-chain antibodies (IgNAR, 'immunoglobulin new antigen receptor'), from which single-domain antibodies called V fragments can be obtained. An alternative approach is to split the dimeric variable domains from common immunoglobulin G (IgG) from humans or mice into monomers. Although most research into single-domain antibodies is currently based on heavy chain variable domains,
derived from light chains have also been shown to bind specifically to target epitopes.
The fusion of a fluorescent protein to a nanobody generates a so-called chromobody. Chromobodies can be used to recognize and trace targets in different compartments of living cells. They can therefore increase the possibilities of live cell microscopy and will enable novel functional studies. The coupling of an anti-GFP Nanobody to a monovalent matrix, called GFP-nanotrap, allows the isolation of GFP-fusion proteins and their interacting partners for further biochemical analyses. Single molecule localization with super-resolution imaging techniques requires the specific delivery of fluorophores into close proximity with a target protein. Due to their large size the use of antibodies coupled to organic dyes can often lead to a misleading signal owing to the distance between the fluorophore and the target protein. The fusion of organic dyes to anti-GFP
targeting GFP-tagged proteins allows nanometer spatial resolution and minimal linkage error because of the small size and high affinity.
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